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Literature summary for 2.3.1.256 extracted from

  • Varland, S.; Myklebust, L.M.; Goksoeyr, S.O.; Glomnes, N.; Torsvik, J.; Varhaug, J.E.; Arnesen, T.
    Identification of an alternatively spliced nuclear isoform of human N-terminal acetyltransferase Naa30 (2018), Gene, 644, 27-37 .
    View publication on PubMed
Search for more literature for 2.3.1.256

Cloned(Commentary)

Cloned (Comment) Organism
gene NAA30, DNA and amino acid sequence, analysis and comparison, alternative splicing variants NAA30_v1 (Naa30362) and NAA30_v2 (Naa30288), cloning from HeLa cells, overexpression of MBP-tagged Naa30362-V5 and Naa30288-V5 in HeLa cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information identification of a splice variant of human NAA30, which encodes a truncated protein named Naa30288. DNA sequencing reveals that the 900 bp product derived from alternative splicing results in a 222 bp deletion from the 3' end of exon 1 (c.550_771del). The truncated splice variant (867 bp) is otherwise identical to NAA30. Splicing NAA30_v2 translates into a truncated isoform (p.Val184_Leu257del) consisting of 288 amino acids (Naa30288). The splice variant is abundantly present in thyroid cancer tissues and in several different human cancer cell lines. Naa30288 localizes predominantly to the nucleus, as opposed to annotated Naa30 which has a cytoplasmic localization. Naa30288 is missing elements of the GNAT fold. Full-length Naa30 acetylates the classical NatC substrate peptide MLGTG in vitro, whereas no significant NAT activity is detected for Naa3028. Naa30288 appears to be enzymatically inactive. Overexpression of full-length Naa30362 increases cell viability via inhibition of apoptosis. In contrast, Naa30288 does not exert an anti-apoptotic effect Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm full-length Naa30 (Naa30362) Homo sapiens 5737
-
additional information immunohistochemic detection in subcellular fractions Homo sapiens
-
-
nucleus alternatively spliced nuclear isoform Naa30288 Homo sapiens 5634
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q147X3 full-length splicing variant Naa30362
-

Purification (Commentary)

Purification (Comment) Organism
subcellular fractionation of HeLa cells and identification of splicing variants of Naa30 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
8305C cell
-
 Homo sapiens
-
A-431 cell
-
 Homo sapiens
-
ARO cell
-
 Homo sapiens
-
B-CPAP cell
-
 Homo sapiens
-
BHT-101 cell
-
 Homo sapiens
-
CAL-62 cell
-
 Homo sapiens
-
CGTH-W1 cell
-
 Homo sapiens
-
FTC-133 cell
-
 Homo sapiens
-
HEK-293 cell
-
 Homo sapiens
-
HeLa-S3 cell
-
 Homo sapiens
-
MCF-7 cell
-
 Homo sapiens
-
NPA cell
-
 Homo sapiens
-
Nthy-ori 3-1 cell
-
 Homo sapiens
-
ONCO-DG1 cell
-
 Homo sapiens
-
peripheral blood mononuclear cell primary Homo sapiens
-
SH-SY5Y cell
-
 Homo sapiens
-
T-84 cell
-
 Homo sapiens
-
TAD-2 cell
-
 Homo sapiens
-
thyroid cancer cell high level of alternatively spliced nuclear isoform Naa30288 Homo sapiens
-
U-87MG cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + N-terminal-L-methionyl-L-alanyl-[PLD] a NatF-type substrate Homo sapiens N-terminal-Nalpha-acetyl-L-methionyl-L-alanyl-[PLD] + CoA
-
 ?
acetyl-CoA + N-terminal-L-methionyl-L-leucyl-[GPE] a NatE-type substrate Homo sapiens N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[GPE] + CoA
-
 ?
acetyl-CoA + N-terminal-L-methionyl-L-leucyl-[GTG] a NatC-type substrate Homo sapiens N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[GTG] + CoA
-
 ?
additional information full-length Naa30 acetylates a classical NatC substrate peptide in vitro, whereas no significant NAT activity is detected for Naa3028. Neither full-length Naa30 nor Naa30288 display any lysine acetyltransferase activity, and neither Naa30 isoforms have KAT activity towards histones. The SESSS, EEEIA and MDELF peptides are negative controls and represent NatA, Naa10 and NatB substrates, respectively. Poor activity with SGRGK peptide, which contains the natural 28-mer N-terminal sequence of histone H4, and represents a NatD-type substrate Homo sapiens ?
-
-

Subunits

Subunits Comment Organism
? x * 84000, recombinant MBP-tagged Naa30362, SDS-PAGE, x * 75000, recombinant MBP-Naa30288, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
N-terminal acetyltransferase Naa30
-
 Homo sapiens
NAA30
-
 Homo sapiens
NatC
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Homo sapiens

General Information

General Information Comment Organism
evolution enzyme Naa30 belongs to the GNAT superfamily of acetyltransferases characterized by the highly conserved GNAT fold, which promotes Ac-CoA binding and substrate recognition Homo sapiens
malfunction overexpression of full-length Naa30 increases cell viability via inhibition of apoptosis. In contrast, Naa30288 does not exert an anti-apoptotic effect Homo sapiens
additional information homology structure modeling of Naa30 using the crystal structure of human Naa50 (PDB ID 3TFY) as template Homo sapiens
physiological function the NatC complex consists of the catalytic subunit Naa30 and the auxiliary subunits Naa35 and Naa38, and can potentially Nt-acetylate cytoplasmic proteins when the initiator methionine is followed by a bulky hydrophobic/amphipathic residue at position 2. Full-length enzyme Naa30362 improves cell viability and inhibits apoptosis Homo sapiens